Effects of corn silage supplementation strategy and grazing intensity on herbage intake, milk production, and behavior of dairy cows.

Effects of corn silage supplementation on milk production of grazing dairy cows depend in part on the substitution rate between the 2 forages, which may be influenced by grazing management. The aim of this study was to compare 2 grazing management strategies for measuring substitution rate between herbage and corn silage, in interaction with grazing intensity. Six treatments were compared, with 2 grazing intensities and 3 supplementation strategies investigated at both grazing intensities. The 2 grazing intensities were severe and light grazing, defined by either (1) herbage allowance (HA) of 15 (severe) or 30 (light) kg dry matter (DM)/cow per d at 3 cm above ground level or (2) postgrazing sward height, depending on the supplementation strategy. The 3 supplementation strategies were as follows: (U) an unsupplemented treatment, (A) 5 kg DM/d of corn silage offered at a similar HA as in U, and (H) 5 kg DM/d of corn silage offered at a similar postgrazing sward height as in U. Thirty-six multiparous Holstein cows were used in a randomized complete block design and divided in 2 groups for the entire experiment, one for each grazing intensity. Within each grazing intensity group, the corn silage supplementation strategy was studied using a 3 × 3 Greco-Latin square design, with 3 periods of 14 d. Supplementing cows with corn silage increased total DM intake only for severe grazing by 1.7 kg DM/d. The substitution rate between corn silage and grazed herbage was lower for severe than for light grazing, averaging 0.63 and 1.23, respectively. Herbage dry matter intake was lower by 1.2 kg/d for strategy H than A, leading to lower substitution rates (0.81 vs. 0.99, respectively), irrespective of grazing intensity. Milk production increased with silage supplementation for severe grazing (+1.0 kg/d milk) and was unaffected by silage supplementation for light grazing (-0.4 kg/d milk). The milk production response to corn silage supplementation averaged +0.23 and -0.08 kg of milk per kg DM of silage for severe and light grazing, respectively. Fat-corrected milk production tended to be lower by 0.4 kg/d for strategy H than A, leading to lower milk production response (+0.00 vs. +0.12 kg of milk per kg DM of silage, respectively). Milk protein concentration increased with silage supplementation for severe grazing (+1.0 g/kg) but decreased with silage supplementation for light grazing (-0.6 g/kg). Milk fat concentration did not differ among treatments. On average, daily grazing time (-47 min/d, i.e., -9%) and herbage intake rate (-4.9 g of DM/min, i.e., -14%) decreased when cows were supplemented, with greater grazing time reduction at severe than light grazing, and greater herbage intake rate reduction at light than severe grazing. In conclusion, the greater substitution rate and the lower 4% fat-corrected milk production when corn silage was provided at a similar postgrazing sward height rather than at a similar HA to those of unsupplemented cows explain why supplementing grazing dairy cows with conserved forages has no strong effect in practice from a production point of view.

Corn silage supplementation for dairy cows grazing annual ryegrass at two pasture allowances / Suplementação com silagem de milho para vacas leiteiras manejadas em duas ofertas diárias em pastagens de azevém anual

Corn silage supplementation for dairy cows grazing in temperate annual pastures has rarely been investigated. The aim of this study is to compare two supplementation levels (0 and 4kg dry matter [DM]/day of a 7:1 mixture of corn silage and soybean meal) in dairy cows strip-grazing annual ryegrass (Lolium multiflorum Lam.) at two pasture allowances (PA, low= 25 and high = 40kg DM/d at ground level). The study was carried out according to an incomplete 4 × 3 Latin square design, using 12 cows and three experimental periods of 12 days. The green leaves allowances were only 4.9 and 8.5kg DM/d at the low and high PA, respectively. The total DM intake and milk production increased in supplemented cows compared to un-supplemented cows at the low PA, but were similar between supplementation levels at the high PA. The PI was unaffected by the PA, whereas the substitution rate was 0.68 in cows at the low PA and 1.35 in cows at the high PA. Corn silage supplementation may improve the total DM intake and milk production of dairy cows grazing in temperate annual pastures, but only at a low PA.(AU)

O objetivo deste estudo foi comparar os efeitos de dois níveis de suplementação (0 e 4kg de matéria seca [MS]/dia de uma mistura de silagem de milho e farelo de soja na razão de 7:1) para vacas leiteiras em pastos de azevém anual (Lolium multiflorum Lam.) manejados em faixas diárias com duas ofertas (OF, baixa= 25 e alta= 40kg de MS/dia medidas em nível do solo). Os tratamentos foram comparados em delineamento quadrado latino incompleto 4 × 3, com 12 vacas em três períodos de 12 dias. A oferta de folhas foi somente de 4,9 e 8,5kg de MS/dia nas OF baixa e alta, respectivamente. O consumo total de MS e a produção de leite aumentaram com a suplementação somente quando os animais estavam em baixa OF, não havendo efeito da suplementação em alta OF. O consumo do pasto não foi afetado pela OF, entretanto a taxa de substituição foi de 0,68 em baixa OF e de 1,35 em alta OF. A suplementação com silagem de milho promove o aumento do consumo total de MS e da produção de leite de vacas manejadas em pastos anuais de clima temperado somente em situações de baixa OF.(AU)

Herbage intake, methane emissions and animal performance of steers grazing dwarf elephant grass v. dwarf elephant grass and peanut pastures.

Management strategies for increasing ruminant legume consumption and mitigating methane emissions from tropical livestock production systems require further study. The aim of this work was to evaluate the herbage intake, animal performance and enteric methane emissions of cattle grazing dwarf elephant grass (DEG) (Pennisetum purpureum cv. BRS Kurumi) alone or DEG with peanut (Arachis pintoi cv. Amarillo). The experimental treatments were the following: DEG pastures receiving nitrogen fertilization (150 kg N/ha as ammonium nitrate) and DEG intercropped with peanut plus an adjacent area of peanut that was accessible to grazing animals for 5 h/day (from 0700 to 1200 h). The animals grazing legume pastures showed greater average daily gain and herbage intake, and shorter morning and total grazing times. Daily methane emissions were greater from the animals grazing legume pastures, whereas methane emissions per unit of herbage intake did not differ between treatments. Allowing animals access to an exclusive area of legumes in a tropical grass-pasture-based system can improve animal performance without increasing methane production per kg of dry matter intake.

Constant time clash detection in protein folding.

Applications for the manipulation of molecular structures are usually computationally intensive. Problems like protein docking or ab-initio protein folding need to frequently determine if two atoms in the structure collide. Therefore, an efficient algorithm for this problem, usually referred as clash detection, can greatly improve the application efficiency. This work focus mainly on the ab-initio protein folding problem. A naive approach for the clash problem, the most commonly-used by molecular structure programs, consists in calculating the distance between every pair of atoms. We propose an efficient data structure that uses a three-dimensional array to store the atoms' position. We compare the proposed data structure with one of the best known general data structures for this type of problems (SAT tree) and with the naive approach. While the naive approach takes linear time to the number of atoms to verify if a new atom clashes with any previously-set atoms, the proposed data structure takes constant time to perform the same verification. The SAT tree takes logarithmic time for the same task. The results show that the proposed data structure surpasses the other techniques for any protein size. The proposed data structure takes near half of the time of the SAT data structure and close to a fifth of the time of the naive approach for the larger proteins. We believe that this data structure can improve the existing molecular structure applications by decreasing the computational cost needed for clash detection. The data structure presented in this work can be used for any protein structure clash verification, as long as the atoms that need to be checked are kept in the 3D array. This data structure is particulary useful when manipulating large sets of atoms, for example, in applications like loop prediction, structure refinement of large proteins, and protein docking.